Antibacterial Efficacy of Silver Diamine Fluoride Compared to Casein Phosphopeptide-Amorphous Calcium Phosphate Against Streptococcus mutans in a Biofilm Caries Model

ABSTRACT Objective: To compare the antibacterial efficacy of silver diamine fluoride (SDF) with a product containing casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) against Streptococcus mutans using a biofilm caries model. Material and Methods: Twenty-seven saliva-coated dentine blocks obtained from extracted human teeth were inoculated with Streptococcus mutans monospecies biofilm in this in vitro study. The biofilms were then exposed to 10% sucrose in brain heart infusion broth eight times daily for seven days. After the biofilm growth period, the dentine blocks (n=9 per group) were treated with one of the following substances: 1) sterile saline (control), 2) 38% SDF, and 3) a product containing CPP-ACP. Then, the samples were incubated at 37ºC for 48 hours, and the numbers of viable microorganisms in the biofilms were counted and compared. ANOVA and Tukey's HSD tests were used to analyze the data (p<0.05). Results: The number of viable bacteria, as determined by the number of colony-forming units (CFU mL-1) of Streptococcus mutans, was significantly reduced following treatment with SDF and the CPP-ACP product (p<0.05). However, SDF showed superior antibacterial activity compared to the CPP-ACP product (mean CFU mL-1 =zero compared to 96 x106) (p<0.05). Conclusion: SDF has higher antibacterial activity against cariogenic Streptococcus mutans biofilm than the CPP-ACP product. The CPP-ACP product showed antibacterial activity, but it was limited.

Comparison of color difference and reliability of two intraoral spectrophotometers

Aim: to evaluate the intra and inter-device reliability of two intraoral spectrophotometers in measuring the Commission Internationale de l'Éclairage (CIE) L*a*b* color coordinates and to compare the color difference (ΔE) between both devices. Methods: the central region of the labial surface of the maxillary central incisor of 31 participants was measured twice by each of the devices (VITA EasyShade and Degudent Shadepilot) by one examiner. CIE L*a*b* color coordinates were obtained for all teeth and ΔE was measured and compared. Intraclass correlation coefficient (ICC) and Mann-whitney U test were used to analyze the data (p<0.05). Results: inter-device reliability ICCs in measuring CIE L*a*b* color coordinates ranged between 0.08-0.49 with significant difference between devices only concerning the b coordinate (p<0.05). While intra device reliability ICCs ranged between 0.86-0.89 for VITA EasyShade and 0.81-0.86 for Degudent Shadepilot. The mean ΔE for CIE L*a*b* color coordinates of VITA EasyShade was 3.61 (±1.93) compared to 3.60 (± 1.45) for Degudent Shadepilot with insignificant difference between both devices (p>0.05). Conclusions: high intra device reliability in measuring CIE L*a*b* color coordinates was achieved particularly of Vita EasyShade, and both devices had clinically acceptable color difference (ΔE <3.7) however, inter device reliability was low to moderate. Consequently, the same spectrophotometer should be used throughout the steps of performing any tooth- colored restoration

Clinical Competence of Undergraduate Dental Students in Pediatric Dentistry at a Saudi Dental School

ABSTRACT Objective: To assess the clinical competence of undergraduate dental students in pediatric dentistry at Qassim University dental school. Material and Methods: A retrospective audit of students' clinical competency sheets of fourth and fifth-year students (n= 102) over two years was performed. Mean competency scores for each clinical procedure aswell as overall scores of the students were compared according to their academic level and gender. Also, the percentage of competent students was compared according to the academic level. Chi-square and t-tests were used to analyze the data (p<0.05). Results: Compared to fifth-year students, fourth-year students were significantly more competent in pediatric dentistry (100% scored >50% overall compared to 86.9 % of fifth-year students). They had significantly higher mean overall scores (84.63 ± 9.15 compared to 67.68 ± 13.83) as well as individual scores in performing an examination, diagnosis, and treatment planning of a child patient, restorations, and stainless steel crowns than fifth-year students. In addition, females had significantly higher mean scores in the aforementioned procedures and in placing esthetic crowns (p<0.05). Conclusion: Fourth-year dental students at Qassim University were more competent clinically than fifth-year students in pediatric dentistry. Also, in most of the shared procedures, females were more competent than males. A deficiency in the competence of fifth-year students was noted in pulp therapy, stainless steel and esthetic crowns procedures, and these are currently being addressed.

What do Parents Know About Oral Health and Care for Preschool Children in the Central Region of Saudi Arabia?

Abstract Objective: To assess the knowledge level of parents from the central region of Saudi Arabia about oral health and care of preschool children and its relation with sociodemographic variables, parents' self-perception toward their dental health, importance of teeth, and frequency of dental visits. Material and Methods: A random sample of 754 parents participated in this cross-sectional study and completed an internationally accepted questionnaire. Chi-square test and logistic regression analysis were used to analyze the data (p<0.05). Results: Mean knowledge score of the parents was 4.8 (out of 11). Less than 20% of the parents were knowledgeable about the best position for tooth brushing, the concentration of fluoride in a child's toothpaste, timing of first dental check-up, and best time to give a sugary snack. Mothers, parents with high educational level and family income, parents with a positive attitude towards teeth and excellent self-perception of their dental health were significantly more likely to score higher (p<0.05). Conclusion: Knowledge of parents about oral health and care of preschool children in the central region of Saudi Arabia was deficient. Gender, education level of parents, family income, attitude about teeth and self-perception of parents' own dental health were factors, which influenced their knowledge. To improve parents' knowledge, health professionals' role should be improved, and TV commercials directed toward the areas, which had gaps in knowledge.